Monday, December 5, 2011

Senses of sophistication: Mosquitoes detect subtle cues finding food, spreading diseases

Senses of sophistication: Mosquitoes detect subtle cues finding food, spreading diseases

Monday, December 5, 2011

Fruit flies and mosquitoes share similar sensory receptors that allow them to distinguish among thousands of sensory cues ? particularly heat and chemical odors ? as they search for food or try to avoid danger, researchers from Boston College and Brandeis University report in the current electronic edition of the journal Nature.

Pinpointing a tiny portion of a protein found on the surfaces of neurons that give the fruit fly Drosophila and the malaria mosquito Anopheles gambiae the ability to distinguish different stimuli, the findings provide new clues that could be used to develop solutions to target disease-spreading mosquitoes.

"Heat is one element of an ensemble of cues we present that mosquitoes read as they search for their next blood meal," said Boston College Professor of Biology Marc A.T. Muskavitch, a co-author of the report. "These findings give us another chance to look at how we might try to reduce the ability of mosquitoes to sense us, and thereby protect ourselves from their bites and the diseases that they spread."

Receptors that sense heat and chemicals are among the ancient biological tools organisms first developed to find food or avoid harm. Over the ages, these receptors have evolved, allowing humans, animals and insects to detect and distinguish among thousands of stimuli. What's puzzled scientists is how animals distinguish between sensory inputs that are detected by the same sensors.

Transient receptor potential (TRP) channels in the brain, skin and other sensory organs play key roles in deciphering thermal, chemical and other sensory cues. But the mechanism a TRP channel uses to distinguish between signals sent by a heat source or by a noxious chemical has eluded researchers.

Building on earlier research into thermosensation in Drosophila by co-author and Brandeis University Professor of Biology Paul A. Garrity, the team found fruit flies possess slightly different versions, or isoforms, of the TRPA1 channel within their neurons. One version of the channel responds to warmth, while the other version responds only to chemicals. The same two TRPA1 isoforms are found in the malaria mosquito Anopheles gambiae, the team reports, giving mosquitoes the ability to distinguish between warmth given off from a potential host and the odor from a chemical repellent.

Muskavitch, whose prior research includes decoding the genes of mosquitoes that transmit human diseases, says finding ways to disrupt the abilities of mosquitoes to sense and bite humans has been a goal of researchers and public health services around the world for some time. Knowing more about the insect's basic responses to its warm-blooded targets and to chemical insecticides and repellents will help inform efforts to reduce the transmission of mosquito-borne illnesses that kill and sicken millions of people around the globe each year.

"If we could somehow make ourselves less 'visible' to the mosquito by reducing its ability to sense our warmth, or the carbon dioxide and other chemicals we emit, we could improve our ability to evade them," said Muskavitch. "The more veils we put between ourselves and mosquitoes, the less likely it is they will bite us and spread diseases among humans."

###

Boston College: http://www.bc.edu

Thanks to Boston College for this article.

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Source: http://www.labspaces.net/115711/Senses_of_sophistication__Mosquitoes_detect_subtle_cues_finding_food__spreading_diseases

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LSU Beats Georgia: 'Honey Badger' Leads No. 1 Tigers To BCS Title Game

ATLANTA -- LSU slogged its way through a brutal first half. The nation's top-ranked team had only 12 yards and not even a single first down.

The "Honey Badger" didn't care.

He just took what he wanted ? a trip to the national championship game.

Tyrann Mathieu turned in an MVP performance when the Tigers needed him most, running back a punt 62 yards for a touchdown, setting up another score with a fumble recovery and finally finishing off No. 12 Georgia with his best play yet, a whirling dervish of a return that led to the decisive TD of a 42-10 victory in the Southeastern Conference championship game Saturday.

LSU (13-0) advanced to a spot in the BCS title game in New Orleans, just 75 miles from its Baton Rouge campus. The Tigers opponent will be announced Sunday night, but SEC West rival and No. 2 Alabama ? already beaten by the Tigers 9-6 in overtime a month ago ? had the inside track even though it didn't win its division.

The Bulldogs tried to really shake things up, racing to a 10-0 lead that could've been even bigger if they hadn't dropped a pair of potential touchdown passes in the first quarter. LSU looked downright awful on offense, going three-and-out on all seven of its possessions before halftime.

But, thanks to Mathieu, the deficit was only 10-7 when the teams went to the locker room. He took a punt at his own 38, found an opening and was gone ? all the way to the end zone for his second punt return for a touchdown in as many weeks. Well, almost to the end zone. A replay showed Mathieu flipped the ball to an official just before he crossed the goal line, but no one on the field caught the blunder.

"Yeah, I kind of felt it," Mathieu said. "I looked at the referee. I'll have to remember not to do that next time."

That was long forgotten by the time the fearless sophomore was done.

"I just tried to make one guy miss and then get to the end zone," Mathieu said.

On Georgia's first possession of the second half, quarterback Aaron Murray tried to scramble for a first down but had the ball knocked loose just before he hit the turf. Who was there to fall on it at the Bulldogs 26?

Mathieu, of course, his fifth fumble recovery of the season.

LSU quickly seized its first lead. The Tigers finally picked up a first down, then freshman Kenny Hilliard broke off a 15-yard run for the first of his three touchdowns. Normally, that would've been more than enough to win the game's MVP award. Not even close on this day.

Mathieu, whose nickname comes from a humorous YouTube video about supposedly the world's more fearless animal ("Honey Badger don't care, he just takes what he wants," the narrator says), dropped back to receive another punt. About the only thing the Bulldogs managed to do was keep him out of the end zone.

The 5-foot-9, 175-pounder cradled the ball, took off down the center of the field, cut back to his left, stutter-stepped and turned on a burst of speed, basically came to a stop around the Georgia 30, then took off again and was finally dragged down at the 17.

He avoided or broke away from at least eight of the 11 red-clad guys trying to bring him down, a Heisman-worthy play that should be enough to at least get him to New York for the banquet ? if not earn him serious consideration for the award as the nation's top player. Certainly, no defensive player has come up with more big plays.

"When you play as hard as he does, good things happen to you," Georgia coach Mark Richt said. "To be honest with you, I enjoy watching him play football except when he plays against us. When you see a guy like that, you appreciate the way he plays. There's just something about him. He finds a way to do something special just about every game. He did it again today."

Mathieu has scored four touchdowns this season: Two on returns, two more from his cornerback spot. He's forced six fumbles. He's picked off two passes. He one of the top tacklers on one of the nation's top defenses.

LSU took control with a 21-point third quarter, coming back from a double-digit deficit for the second week in a row and leaving little doubt that it's the best team in country heading into bowl season. The only other unbeaten team, Houston, was blown out in the Conference USA championship game Saturday. All the other top teams have at least one loss.

If there was ever a year when no playoff was needed, it's this one. The Tigers have knocked off five teams in The Associated Press' current Top 25 ? including three of the top eight. They'll still have to win one more game to claim the BCS title, but a case can be made that they deserve to be voted No. 1 in the AP poll, no matter what happens Jan. 9 in the Big Easy.

SEC East champion Georgia came into the game on a 10-game winning streak, and the Bulldogs showed no fear of LSU in the early going. Murray connected with Tavarres King on a 44-yard pass and could've had a TD when a pass across the middle went through King's hands. They settled for Blair Walsh's 40-yard field goal.

Knowing he would have to throw caution to the wind to pull off the upset against a 13 1/2-point favorite, Richt called an onside kick. It worked the perfection, Walsh bouncing the ball off the turf and high into the air. Alec Ogletree soared in to grab the ball beyond the necessary 10 yards, giving the Bulldogs another possession.

They should've scored a touchdown. Instead, they wound up with nothing. Freshman Malcolm Mitchell dropped a pass right in his hands at the LSU 5 with no one around, and Walsh missed a 45-yard try.

But LSU couldn't do anything with the ball. The Tigers' longest play in the first half was 9 yards. Eleven of their 21 plays went for zero or negative yards. Outside of Mathieu, punter Brad Wing was LSU's best field-turning weapon, averaging 54.1 yards on his first seven punts.

Both offenses stalled in the second quarter, managing a grand total of 2 yards ? 1 for each team. Georgia went to the locker room with a commanding 135-12 lead in total yards, but certainly a sense it had missed its chance to put away an LSU team that had outscored No. 6 Arkansas 41-3 after falling into an early 14-0 hole the previous week.

Hilliard scored on a 4-yard run after Mathieu's second big return to make it 21-10, essentially enough to finish off the Bulldogs, but he then hauled in a 8-yard touchdown pass.

The Tigers romped in the final period. Alfred Blue broke off a 48-yard touchdown run and Morris Claiborne returned an interception 45 yards for the final score. LSU won even though Jordan Jefferson completed only five passes and the offense totaled a mere 237 yards.

Murray had a miserable day, completing just 16 of 40 for 163 yards with two interceptions. Georgia's running game was non-existent with Isaiah Crowell hobbling on a sore ankle. The freshman had only 15 yards on 10 carries.

___

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Source: http://www.huffingtonpost.com/2011/12/03/lsu-beats-georgia-honey-badger-football-sec-bcs_n_1127638.html

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Gingrich Leads in Des Moines Register Poll (ABC News)

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LSU Beats Georgia: 'Honey Badger' Leads No. 1 Tigers To BCS Title Game

ATLANTA -- LSU slogged its way through a brutal first half. The nation's top-ranked team had only 12 yards and not even a single first down.

The "Honey Badger" didn't care.

He just took what he wanted ? a trip to the national championship game.

Tyrann Mathieu turned in an MVP performance when the Tigers needed him most, running back a punt 62 yards for a touchdown, setting up another score with a fumble recovery and finally finishing off No. 12 Georgia with his best play yet, a whirling dervish of a return that led to the decisive TD of a 42-10 victory in the Southeastern Conference championship game Saturday.

LSU (13-0) advanced to a spot in the BCS title game in New Orleans, just 75 miles from its Baton Rouge campus. The Tigers opponent will be announced Sunday night, but SEC West rival and No. 2 Alabama ? already beaten by the Tigers 9-6 in overtime a month ago ? had the inside track even though it didn't win its division.

The Bulldogs tried to really shake things up, racing to a 10-0 lead that could've been even bigger if they hadn't dropped a pair of potential touchdown passes in the first quarter. LSU looked downright awful on offense, going three-and-out on all seven of its possessions before halftime.

But, thanks to Mathieu, the deficit was only 10-7 when the teams went to the locker room. He took a punt at his own 38, found an opening and was gone ? all the way to the end zone for his second punt return for a touchdown in as many weeks. Well, almost to the end zone. A replay showed Mathieu flipped the ball to an official just before he crossed the goal line, but no one on the field caught the blunder.

"Yeah, I kind of felt it," Mathieu said. "I looked at the referee. I'll have to remember not to do that next time."

That was long forgotten by the time the fearless sophomore was done.

"I just tried to make one guy miss and then get to the end zone," Mathieu said.

On Georgia's first possession of the second half, quarterback Aaron Murray tried to scramble for a first down but had the ball knocked loose just before he hit the turf. Who was there to fall on it at the Bulldogs 26?

Mathieu, of course, his fifth fumble recovery of the season.

LSU quickly seized its first lead. The Tigers finally picked up a first down, then freshman Kenny Hilliard broke off a 15-yard run for the first of his three touchdowns. Normally, that would've been more than enough to win the game's MVP award. Not even close on this day.

Mathieu, whose nickname comes from a humorous YouTube video about supposedly the world's more fearless animal ("Honey Badger don't care, he just takes what he wants," the narrator says), dropped back to receive another punt. About the only thing the Bulldogs managed to do was keep him out of the end zone.

The 5-foot-9, 175-pounder cradled the ball, took off down the center of the field, cut back to his left, stutter-stepped and turned on a burst of speed, basically came to a stop around the Georgia 30, then took off again and was finally dragged down at the 17.

He avoided or broke away from at least eight of the 11 red-clad guys trying to bring him down, a Heisman-worthy play that should be enough to at least get him to New York for the banquet ? if not earn him serious consideration for the award as the nation's top player. Certainly, no defensive player has come up with more big plays.

"When you play as hard as he does, good things happen to you," Georgia coach Mark Richt said. "To be honest with you, I enjoy watching him play football except when he plays against us. When you see a guy like that, you appreciate the way he plays. There's just something about him. He finds a way to do something special just about every game. He did it again today."

Mathieu has scored four touchdowns this season: Two on returns, two more from his cornerback spot. He's forced six fumbles. He's picked off two passes. He one of the top tacklers on one of the nation's top defenses.

LSU took control with a 21-point third quarter, coming back from a double-digit deficit for the second week in a row and leaving little doubt that it's the best team in country heading into bowl season. The only other unbeaten team, Houston, was blown out in the Conference USA championship game Saturday. All the other top teams have at least one loss.

If there was ever a year when no playoff was needed, it's this one. The Tigers have knocked off five teams in The Associated Press' current Top 25 ? including three of the top eight. They'll still have to win one more game to claim the BCS title, but a case can be made that they deserve to be voted No. 1 in the AP poll, no matter what happens Jan. 9 in the Big Easy.

SEC East champion Georgia came into the game on a 10-game winning streak, and the Bulldogs showed no fear of LSU in the early going. Murray connected with Tavarres King on a 44-yard pass and could've had a TD when a pass across the middle went through King's hands. They settled for Blair Walsh's 40-yard field goal.

Knowing he would have to throw caution to the wind to pull off the upset against a 13 1/2-point favorite, Richt called an onside kick. It worked the perfection, Walsh bouncing the ball off the turf and high into the air. Alec Ogletree soared in to grab the ball beyond the necessary 10 yards, giving the Bulldogs another possession.

They should've scored a touchdown. Instead, they wound up with nothing. Freshman Malcolm Mitchell dropped a pass right in his hands at the LSU 5 with no one around, and Walsh missed a 45-yard try.

But LSU couldn't do anything with the ball. The Tigers' longest play in the first half was 9 yards. Eleven of their 21 plays went for zero or negative yards. Outside of Mathieu, punter Brad Wing was LSU's best field-turning weapon, averaging 54.1 yards on his first seven punts.

Both offenses stalled in the second quarter, managing a grand total of 2 yards ? 1 for each team. Georgia went to the locker room with a commanding 135-12 lead in total yards, but certainly a sense it had missed its chance to put away an LSU team that had outscored No. 6 Arkansas 41-3 after falling into an early 14-0 hole the previous week.

Hilliard scored on a 4-yard run after Mathieu's second big return to make it 21-10, essentially enough to finish off the Bulldogs, but he then hauled in a 8-yard touchdown pass.

The Tigers romped in the final period. Alfred Blue broke off a 48-yard touchdown run and Morris Claiborne returned an interception 45 yards for the final score. LSU won even though Jordan Jefferson completed only five passes and the offense totaled a mere 237 yards.

Murray had a miserable day, completing just 16 of 40 for 163 yards with two interceptions. Georgia's running game was non-existent with Isaiah Crowell hobbling on a sore ankle. The freshman had only 15 yards on 10 carries.

___

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Source: http://www.huffingtonpost.com/2011/12/03/lsu-beats-georgia-honey-badger-football-sec-bcs_n_1127638.html

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Michael Jackson Doctor Conrad Murray Files Appeal

Murray will represent himself as he contests involuntary manslaughter conviction for which he faces up to four years in state prison.
By Kara Warner


Dr. Conrad Murray
Photo: Pool/ Getty Images

As expected, Michael Jackson's former doctor Conrad Murray has filed an appeal to challenge his involuntary manslaughter conviction.

According to TMZ, Murray filed the petition in pro per, which means he has decided to forego hiring legal representation and represent himself. He reportedly listed "Men's Central Jail" and his booking number in the space on the appeal document where a lawyer would normally list a firm's name or contact information.

TMZ also reported that although Murray seems to be without a lawyer, he is still in communication with Nareg Gourjian, one of the attorneys who represented him during his manslaughter trial.

"This case presents a gold mine of issues for any appellate lawyer," Gourjian told TMZ. "Dr. Murray is confident that the Court of Appeal will vindicate him."

Following the guilty verdict, Murray's lead defense lawyer Ed Chernoff told CNN, "What matters most right now is trying to keep Dr. Murray from taking up a prison cell in this community. That's what we're focusing on right now, and we'll deal with an appeal after that."

Murray was sentenced to four years in jail Tuesday, but due to overcrowding, he's unlikely to serve his full term.

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Source: http://www.mtv.com/news/articles/1675343/conrad-murray-conviction-appeal.jhtml

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Sunday, December 4, 2011

PFT: Peyton improving, will increase rehab

Peyton ManningAP

Peyton Manning says he?s getting better at a rather important part of playing quarterback ? throwing the football ? as he continues his recovery from neck surgery.

?We have some more questions to answer,? Manning told reporters in the Colts? locker room today, one day after the Colts announced that he had moved to the next phase in his rehab. ?We have some things I haven?t been allowed to do. Now we can do a little more. Throwing ? that?s what you?re asking me ? yeah, throwing will be part of this next progression. That is critical to my job. So, yeah, I will be throwing and have been doing some throwing and we?ve got to ramp that up a little bit and answer some questions about where we are.?

The mere fact that Manning can throw a football is a positive step, although he?s not at the point where he?s practicing with the Colts, and it still sounds like a long shot that he would actually be able to throw enough to get on the field this season. But Manning was smiling as he spoke and sounded encouraged.

?It?s nice having the reins cut off you a little bit, and having a little more freedom to do weightlifting activities and on-the-field activities that you?re normally used to doing,? Manning said. ?It?s good because it means we?ve done what we?re supposed to do over the past three months.?

Still, Manning says he can?t be sure that his health will continue to progress, and he?s not sure when he?ll be healthy enough to play in an NFL game again.

?It doesn?t guarantee anything in this next phase, but it does allow you to step into that phase,? Manning said. ?The worst news we could have heard yesterday was ?You?re not where you want to be and you?ve got to keep doing what you?re doing.? He has given us clearance for the next phase, and I?ll follow that as well as I can.?

And he can only hope that he continues to improve to the point where he throws as well as ever next year, with the Colts or some other team.

Source: http://profootballtalk.nbcsports.com/2011/12/01/peyton-manning-moves-to-next-phase-of-rehab-with-no-timetable-for-a-return/related/

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American denies involvement in Aruba disappearance

Gary Giordano of Gaithersburgh, Md., who was detained for four months after the presumed death of his traveling companion, Robyn Gardner, in Aruba, pauses during an interview on ABC television's "Good Morning America" in New York, Thursday, Dec. 1, 2011. During the interview Giordano, 50, refused to detail what happened to Gardner on Aug. 2, the last day she was seen alive, saying he has told that story to investigators "50, 60 times." He has said she was swept out to sea while snorkeling. Her body has not been found. (AP Photo/Ida Mae Astute, ABC)

Gary Giordano of Gaithersburgh, Md., who was detained for four months after the presumed death of his traveling companion, Robyn Gardner, in Aruba, pauses during an interview on ABC television's "Good Morning America" in New York, Thursday, Dec. 1, 2011. During the interview Giordano, 50, refused to detail what happened to Gardner on Aug. 2, the last day she was seen alive, saying he has told that story to investigators "50, 60 times." He has said she was swept out to sea while snorkeling. Her body has not been found. (AP Photo/Ida Mae Astute, ABC)

Gary Giordano, right, of Gaithersburgh, Md., who was detained for four months after the presumed death of his traveling companion, Robyn Gardner, in Aruba, is interviewed by Robin Roberts on ABC television's "Good Morning America" in New York, Thursday, Dec. 1, 2011. During the interview Giordano, 50, refused to detail what happened to Gardner on Aug. 2, the last day she was seen alive, saying he has told that story to investigators "50, 60 times." He has said she was swept out to sea while snorkeling. Her body has not been found. (AP Photo/Ida Mae Astute, ABC)

Gary Giordano, center, a Maryland businessman who had been jailed in Aruba on suspicion of involvement in the presumed death of his traveling companion, walks through Miami International Airport in Miami with attorney Jose Baez, right, after leaving Aruba, Wednesday, Nov. 30, 2011. Giordano denies any wrongdoing in the disappearance of Robyn Gardner. A judge ordered his release after ruling prosecutors didn't have enough evidence to justify holding him longer. (AP Photo/Wilfredo Lee)

Gary Giordano, a Maryland businessman who had been jailed in Aruba on suspicion of involvement in the presumed death of his traveling companion, walks through Miami International Airport in Miami after leaving Aruba, Wednesday, Nov. 30, 2011. Giordano denies any wrongdoing in the disappearance of Robyn Gardner. A judge ordered his release after ruling prosecutors didn't have enough evidence to justify holding him longer. (AP Photo/Wilfredo Lee)

Gary Giordano, left, a Maryland businessman who had been jailed in Aruba on suspicion of involvement in the presumed death of his traveling companion, walks through Miami International Airport in Miami with attorney Jose Baez, right, after leaving Aruba, Wednesday, Nov. 30, 2011. Giordano denies any wrongdoing in the disappearance of Robyn Gardner. A judge ordered his release after ruling prosecutors didn't have enough evidence to justify holding him longer. (AP Photo/Wilfredo Lee)

(AP) ? An American businessman suspected in the presumed death of his traveling companion in Aruba said Thursday that he had nothing to do with her disappearance.

Appearing on ABC television's "Good Morning America," Gary Giordano defended his actions in the wake of Robyn Gardner's disappearance, criticized his former attorney and said the Aruban economy was driven by cocaine and human trafficking.

Later Thursday, the chief Aruban prosecutor said Giordano remains the chief suspect in Gardner's death and that the investigation remains active.

The interview was Giordano's first since he was released Tuesday from an Aruban jail. He flew Wednesday to the United States, and after he landed, an appeals court ruled that prosecutors lacked sufficient evidence to continue holding him. Later Wednesday, the 50-year-old employment services company owner was reunited with his three sons.

In an 11-minute interview with "Good Morning America" anchor Robin Roberts, Giordano, 50, refused to detail what happened to Gardner on Aug. 2. That was the last day she was seen alive, and he said he has told that story to investigators "50, 60 times." He has said she was swept out to sea while snorkeling with him. Her body has not been found.

Asked whether he had anything to do with Gardner's disappearance, Giordano said, "absolutely not."

But he said he does have regrets.

"A person that I cared about, a companion ... has disappeared on my watch," he said. "It will weigh heavily on me for a very long time."

The case recalled that of Alabama teenager Natalee Holloway, who disappeared in May 2005 on a high school graduation trip to Aruba. Her body also was never found and the prime suspect, Joran van der Sloot, was detained for months before being released for lack of evidence. He is now facing trial in the death of a woman in Peru.

Aruban Solicitor General Taco Stein said authorities would ask for Giordano's extradition as soon as they are ready to bring charges. But with no direct evidence that a crime was even committed, there is no guarantee that charges will be filed. The FBI is assisting with the probe, and Stein said he could not say when it would conclude.

Giordano's attorneys have said he likely cannot be returned to Aruba unless prosecutors intend to bring him to trial.

Stein said the interview did not change his opinion that Giordano is the main suspect.

"Clearly, if you look at what he said there, he is avoiding the subject and not making clear statements about what happened," Stein said.

Giordano tried to clarify aspects of his behavior that investigators have called suspicious. He said the $1.5 million accidental death policy he took out on Gardner was part of a travel insurance package that covered both of them. Giordano, who is divorced, said he always takes out travel insurance because he wants his sons to be protected if something happens to him.

"If I go traveling and I disappear, I want them to be covered. I maxed out on everything," Giordano said.

He said he inquired about the insurance two days after Gardner's disappearance on the advice of his former attorney, Michael Lopez. He said Lopez told him he could be billed for expenses related to the search for Gardner.

Prosecutors have mentioned the insurance policy as a possible motive in Gardner's death.

Giordano denied that he and Gardner had been drinking before they went snorkeling. "We were a sober couple," he said.

He also said surveillance video of him in the immediate aftermath of Gardner's disappearance fails to capture his sense of urgency. The video shows him shirtless, wearing swim trunks and tennis shoes, knocking on doors. He does not appear panicked.

He said he was exhausted after swimming back to shore and couldn't find anyone.

"There's nobody there! I'm supposed to scream into the air?" Giordano said.

Giordano was arrested Aug. 5 as he was preparing to board his previously scheduled flight out of Aruba. He said he had been told by authorities that he could leave and that Gardner's mother also encouraged him to go home.

Gardner's friends and family have expressed doubts about her disappearance, saying it was unlikely the 35-year-old woman would have gone snorkeling in the first place. Her mother did not immediately return a message Thursday.

At the end of the interview, Giordano asserted that cocaine and human trafficking were Aruba's "two main sources of income," although he did not explicitly suggest that either had anything to do with Gardner's disappearance. He also noted that Venezuela is a 30-minute boat ride away from the beach where he and Gardner went snorkeling.

Stein was perplexed by Giordano's comments.

"There has been no mention of drugs in this case," Stein said. "It's rather odd for Mr. Giordano to mention this because it contradicts his statements. He said they went snorkeling and the weather got rough and she didn't come out of the water. Did he see a boat? He contradicts himself that way."

Giordano spoke alongside his attorney, Jose Baez, who won an acquittal earlier this year for Florida mother Casey Anthony, who was accused of killing her daughter. Baez was critical of Giordano's former attorney, Michael Lopez, saying that Lopez gave his client bad legal advice and was driven by greed.

In a statement, Lopez said Baez's comments "are not based on reality."

Giordano's sons ? Jason, 19, and twins Tyler and Joshua, 14 ? stood behind him during the interview. After he found out Wednesday that the prosecutors' appeal was denied, he told ABC News that he never expected to be freed.

"I'd accepted the fact that they were gonna keep me from my kids forever," Giordano said.

___

Associated Press writer Ben Fox in San Juan, Puerto Rico, contributed to this report.___Follow Ben Nuckols on Twitter at http://twitter.com/APBenNuckols .

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/386c25518f464186bf7a2ac026580ce7/Article_2011-12-01-Aruba-Missing%20American/id-219e449f7f214c5f8dd4cee634825972

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